头花龙胆组织培养繁殖技术
作者：郭美 刘欣颖 卢虹 梁露 孙爱群 林文敏
来源：《安徽农学通报》2017年第13期

        摘 要：为获得优良健壮的组培苗，建立头花龙胆的无性快繁体系，以六盘水采头花龙胆茎段为外植体，进行愈伤组织的诱导与分化、芽的增殖与继代、根的分化培养。结果表明：MS+NAA0.2mg/L+6BA0.4mg/L培养基适宜诱导，诱导率为93.3%；MS+NAA0.1mg/L+6BA0.3mg/L培养基适宜增殖分化，增殖系数达10.2；MS+NAA0.1mg/L+6BA0.1mg/L培养基适宜生根，接种30d即可生根，生根率100%，同时也适宜增殖。

        关键词：头花龙胆；组织培养；快速繁殖；茎段

        中图分类号 Q946.5 文献标识码 A 文章编号 1007-7731（2017）13-0034-03

        Culture and Rapid Propagation of Tissue of Gentiana Cephantha

        Guo Mei et al.

        （Department of Biology Science，Liupanshui Normal University，Liupanshui 553004，China）

        Abstract：In order to obtain excellent robust plantlets of Gentiana cephantha，the asexual rapid propagation system of Gentiana cephantha was established. Stems as explants，induction and differentiation of callus，bud proliferation and subculture，differentiation of culture root were studied. The results showed that MS+NAA0.2mg/L+6BA0.4mg/L medium was suitable for induction，the induction rate was 93.3%；MS+NAA0.1mg/L+6BA0.3mg/L culture base was suitable for proliferation and differentiation，proliferation coefficient was 10.2. MS+NAA0.1mg/L+6BA0.1mg/L was the suitable culture medium for rooting，and the culture medium was also suitable for proliferation.